Fig. 3

Increased CD8⁺ Tfh and GC B cells in mice with CD8-specific deficiency of Stat5. a Stat5 deficiency in CD8⁺ T cells markedly increases the percentage of CXCR5⁺PD-1⁺CD8⁺ T cells in Ig^HELsHEL transgenic mice. Splenocytes from Stat5^+/+CD8CreIg^HELsHEL and Stat5^fl/−CD8CreIg^HELsHEL mice were stained with anti-CD4, anti-CD8, anti-CD44, anti-CXCR5 and anti-PD-1 antibodies. Numbers indicate percentages of CXCR5⁺PD-1⁺ cells in the gated CD4⁺ or CD8⁺ population (upper and lower left) or CXCR5⁺PD-1⁺CD8⁺ T cell numbers (lower right) and each dot represents an individual mouse (lower). Mean ± SD is shown. b Increased GC B cells in Stat5^fl/−CD8Cre mice following acute LCMV infection. Stat5^+/−CD8Cre and Stat5^fl/−CD8Cre mice were infected with LCMV. Eight days after infection, splenocytes were stained with anti-B220, anti-Fas and anti-GL7 antibodies. Numbers indicate percentages of Fas⁺GL7⁺ cells in the gated B220⁺ population (left) and each dot represents an individual mouse (right). Mean ± SD is shown. c Markedly increased CXCR5⁺PD-1⁺CD8⁺ T cells in Stat5^fl/−CD8Cre mice following acute LCMV infection. Splenocytes from the LCMV-infected mice were also stained with anti-CD4, anti-CD8, anti-CXCR5 and anti-PD-1 antibodies. Numbers indicate percentages of CXCR5⁺PD-1⁺ cells in the gated CD4⁺ or CD8⁺ population (left and middle) or CXCR5⁺PD-1⁺CD8⁺ T cell numbers (right) and each dot represents an individual mouse (middle and right). Mean ± SD is shown. P-values were calculated with the unpaired two-tailed Student’s t-test. Data shown are obtained from 9 (a) Stat5^+/+CD8Cre/YFPIg^HELsHEL and 10 (a) Stat5^fl/−CD8Cre/YFPIg^HELsHEL, 7 (b, c) Stat5^+/−CD8Cre/YFP, and 6 (b) or 8 (c) Stat5^fl/−CD8Cre/YFP mice. Source data are available as a source data file