Fig. 3

Meiotic arrest at Pachytene stage in Uhrf1 cKO testes. a Co-immunofluorescent staining for the DDX4 (germ cell marker, red) and the PLZF (undifferentiated spermatogonial marker, green) on control and Uhrf1 cKO mice at P7, 10, and 14 is shown. Nuclei were stained with DAPI. The right histograms analyzed the quantifications of PLZF⁺ cells per tubule and DDX4⁺ cells per tubule at P7, 10, and 14 in Ctrl (control) and Uhrf1 cKO testes. Mean ± SEM, n = 3–7. *P < 0.05 by Student’s t test. Scale bar = 100 μm. Source data are provided as a source data file. b Co-immunofluorescent staining for the UHRF1 and STRA8 on control and Uhrf1 cKO testis sections at P10, P18, and P56 are shown. Nuclei were stained with DAPI. Scale bar = 100 μm. c Co-immunofluorescent staining for the γ-H2A.X (red) and the SYCP3 (green) on control and Uhrf1 cKO testis sections at P10 and P14 are shown. Nuclei were stained with DAPI (blue). Scale bar = 100 μm. d Labeling of spermatocyte spreads from control and Uhrf1 cKO testes at P21 stained with SYCP3 are shown. Scale bar = 5 μm. e Immunofluorescent staining with anti-SYCP3 and anti-γ-H2A.X on surface-spread pachytene spermatocytes from control and Uhrf1 cKO mice are shown. Scale bar = 5 μm. f Immunofluorescent staining with anti-SYCP3 and anti-RPA2 on surface-spread pachytene spermatocytes from control and Uhrf1 cKO mice are shown. Scale bar = 5 μm