Fig. 6
From: UHRF1 suppresses retrotransposons and cooperates with PRMT5 and PIWI proteins in male germ cells
UHRF1 co-localizes with PRMT5 and regulates histone arginine methylations in spermatocytes. a Co-immunofluorescent staining with UHRF1 (red) and the PRMT5 (green) on control and Uhrf1 cKO testes at P14, P21, and P56. Nuclei were stained with DAPI (blue). Scale bar = 50 μm. b Co-immunoprecipitation of PRMT5 followed by western blot detection of UHRF1 and MIWI from adult control and Uhrf1 cKO testes. c Co-immunofluorescent staining for the γ-H2A.X with PRMT5, H4R3me2s, H3R2me2s, respectively, in P18 control and Uhrf1 cKO testes. Nuclei were stained with DAPI (blue). Scale bar = 25 μm. d Co-Immunofluorescent staining with PRMT5 and SYCP3 of surface-spread pachytene spermatocytes from control and Uhrf1 cKO testes. Scale bar = 5 μm. e Western blot dection of UHRF1 and PRMT5 protein levels in NIH3T3 cells with treatment of GSK591 (an inhibitor of PRMT5) in different concentration for 24 and 48 h. f, g Histogram showing the quantification of UHRF1 and PRMT5 levels in (e). *P < 0.05 by Student’s t-test. Data are presented as mean ± SEM, n = 3. Source data are provided as a source data file. h Co-Immunofluorescent staining with H4R3me2s and SYCP3 of surface-spread pachytene spermatocytes from control and Uhrf1 cKO testes. Scale bar = 5 μm
