Fig. 7
From: UHRF1 suppresses retrotransposons and cooperates with PRMT5 and PIWI proteins in male germ cells
UHRF1 interacts with PRMT5 and regulates gene expression in testes. a The schematic illustration of the distinct domains of UHRF1 and PRMT5. b UHRF1 interacts with PRMT5. HEK293T cells were transfected with indicated plasmids. After 48 h transfection, immunoprecipitation was performed using anti-MYC antibody. FLAG-tagged UHRF1 and MYC-tagged PRMT5 were detected by western blotting using anti-FLAG and anti-MYC antibodies. c The TTD domain of UHRF1 interacts with the SAM domain of PRMT5. HEK293T cells were transfected with indicated plasmids. Forty-eight hours after transfection, immunoprecipitation was performed using anti-MYC antibody. FLAG-tagged UHRF1 fragments and MYC-tagged PRMT5 SAM domain were detected by western blotting using anti-FLAG and anti-MYC antibodies. d RNA-seq analysis of retrotransposon repeat elements using Repbase. Fourteen repeat elements were derepressed in P12 testes of Uhrf1 cKO (shown as red dots; FDR < 0.05, Wald test and > 2 fold change). LINEs are shown in red font, and IAPs are shown in blue font. Datasets for L1Mdtf1 and L1MdtfII are overlapped (shown in a blue circle). e Graph plot showing deregulated genes in Uhrf1 cKO testes at P12. Significantly regulated genes have a P-value of < 0.05 and fold change of > 2. Three biological replicates were indicated. f Gene ontology term analyses of the 213 deregulated genes from WT control and Uhrf1 cKO testes at P12
