Fig. 5 | Nature Communications

Fig. 5

From: Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules

Fig. 5

DOX-inducible GFP-reporter cells to quantify drug transfer. We used the parallel drop maker to generate homogenous mixtures in which 50% of the droplets contained DOX (at the indicated concentrations), 42% contained ≥1 cell, and 8% were empty. We incubated droplets at 37 °C for the indicated times, isolated the cells from the droplets, and quantified the GFP+ cells using flow cytometer. GFP intensity is plotted against forward scatter-area (FSC-A). For the positive control in bulk, we cultured the DOX-GFP-HEK 293 cells with DOX in six well plates using standard cell culture methods. For the Positive Control (Droplet), all droplets, including those with cells, contained DOX at the indicated concentration

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