Fig. 1
The RSV genome and antigenome/mRNAs are m6A methylated. a Distribution of m6A peaks in the RSV antigenome and genome of virions grown in HeLa cells. Confluent HeLa cells were infected by rgRSV at an MOI of 1.0, supernatant was harvested at 36 h post-infection. RSV virions were purified by sucrose gradient ultracentrifugation. Total RNAs were extracted from purified virions and were subjected to m6A-specific antibody immunoprecipitation followed by high-throughput sequencing (m6A-seq). A schematic diagram of partial RSV antigenome (complementary to regions from the leader sequence to M2-2 gene) is shown, as most m6A peaks are clustered in these regions. m6A sites in full-length antigenome and genome are shown in Supplementary Fig. 2. The normalized coverage from m6A-seq of RSV RNA showing the distribution of m6A-immunoprecipitated (IP) reads mapped to the RSV antigenome (blue block) and genome (pink block). The baseline distributions for antigenome and genome from input sample are shown as a blue and pink line respectively. Data presented are the averages from two independent virion samples (n = 2). b Distribution of m6A peaks in the RSV mRNAs from RSV-infected HeLa cells. Confluent HeLa cells were infected by rgRSV at an MOI of 1.0, cell lysates were harvested at 36 h post-infection. Total RNAs were extracted from cell lysates, and were enriched for mRNA by binding to oligo dT, and subjected to m6A-seq. The distribution of m6A-immunoprecipitated (IP) reads were mapped to the RSV mRNAs (pink block). The baseline distributions for mRNAs from input sample are shown as a pink line. Data presented are the averages from two independent virus-infected HeLa cell samples (n = 2). c Distribution of m6A peaks in the RSV antigenome and genome of virions grown in A549 cells. Data presented are the averages from two independent virion samples (n = 2). d Distribution of m6A peaks in the RSV mRNAs from RSV-infected A549 cells. Data presented are the averages from two independent virus-infected A549 cell samples (n = 2)
