Fig. 3
Knockdown of endogenous YTHDF1, 2, 3 (reader) proteins diminishes RSV gene expression. HeLa cells were transfected with 150 pmole of siRNA targeting YTHDF1, 2, 3 or control siRNA. At 36 h post-transfection, cells were infected with rgRSV at an MOI of 0.5. a Immunoblot analysis of YTHDF1, 2, 3 in HeLa cells transfected with siRNA. b Immunoblot analysis of RSV G and F proteins. c Dynamics of GFP expression in YTHDF1, 2, 3 protein-depleted HeLa cells. Micrographs with ×10 magnification (scale bar of 100 μm) are shown. d Quantification of GFP-positive cells by flow cytometry at 18 h post-inoculation. Fold of GFP signal compared to the control is shown. Western blots and GFP images shown are the representatives of three independent experiments. Flow cytometry data are expressed as mean of three independent experiments ± standard deviation. The P value (Student’s t-test) for YTHDF1, 2, and 3 is ****P = 1.10 × 10−6, **P = 0.00170, and ***P = 0.000972, respectively
