Fig. 6
RSV infection does not alter the m6A reader, writer, or eraser protein distribution in cells. HeLa cells were infected by rgRSV at an MOI of 10.0. At 24 h post-infection, mock- or rgRSV-infected cells were stained with anti-reader, writer, or eraser protein antibody (green) and anti-RSV N protein antibody (red), and were analyzed by confocal microscope. Nuclei were labeled with DAPI (blue). Micrographs with ×60 magnification (scale bar of 20 μm) are shown. a m6A reader protein YTHDF2; b m6A writer protein METTL3; and c m6A eraser protein FTO. d Detection of m6A reader, writer, and eraser proteins by Western blot. Nuclear and cytoplasmic fractions were separated from mock- or rgRSV-infected HeLa cells, and were subjected to western blot. Nuclear and cytoplasmic markers were indicated by Lamin A and α-Tubulin, respectively. Representative results from three independent experiments are shown
