Table 1 Baits used in the AP-MS experiments for the characterization of the midbody interactome
From: The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis
Name | Function | Localization | Tag and cell line reference |
|---|---|---|---|
Anillin | Actomyosin binding protein; contractile ring scaffolding | Cleavage furrow, midbody ring and secondary constriction sites | GFP48 |
Aurora B | Serine/threonine kinase; CPC component, controls furrow ingression, central spindle formation and abscission | Cleavage furrow, central spindle, midbody arms | GFP (this study) |
CHMP4B | ESCRT-III protein; required for abscission | Midbody core and abscission site | GFP49 |
CHMP4C | ESCRT-III protein; required for abscission | Central spindle, midbody arms, midbody core and abscission site | Flag26 |
Citron kinase (CIT-K) | Serine/threonine kinase; required for midbody assembly, organization and maturation | Cleavage furrow, central spindle, midbody ring | AcGFP11 |
Ect2 | Rho GEF; activates RhoA to promote contractile ring assembly and constriction | Cleavage furrow, central spindle, midbody core | AcGFP50 |
KIF14 | Kinesin, CIT-K partner; required for midbody assembly, organization and maturation | Cleavage furrow, central spindle, midbody ring | GFP51 |
KIF20A/MKLP2 | Kinesin; required for central spindle formation and CPC translocation | Central spindle, midbody arms | GFP51 |
KIF23/MKLP1 | Kinesin; centralspindlin component, required for furrow ingression, central spindle and midbody formation | Central spindle, midbody core | GFP52 |
PRC1 | Microtubule associated protein, required for central spindle and midbody formation | Central spindle, internal midbody arms and core | GFP53 |
