Fig. 6 | Nature Communications

Fig. 6

From: Rare mutations in the complement regulatory gene CSMD1 are associated with male and female infertility

Fig. 6

C3 and CSMD1 protein colocalize at the oocyte surface. a Spatial relationships between C3 and CSMD1 in developing follicles. C3 and CSMD1 immunofluorescence of oocytes in developing follicles. In most follicles, C3 and CSMD1 colocalize at the oocyte plasma membrane with overlapping signal (Colocalization, left panels). Occasionally the two signals separate and C3 is localized in a tight but distinct ring outside of CSMD1 (no colocalization, right panels). b Spatial relationships between C3 and F4/80-positive macrophages in developing, atretic, and post-ovulatory follicles. F4/80 immunofluorescence of adjacent follicles shows positive macrophage signal in corpus luteum, but not within developing follicles. C3 is localized to the oocyte membrane, as well as in the follicular fluid of the developing antrum (asterisk). Atretic follicles at different stages of degeneration (white arrows) show varying levels of C3 and F4/80 expression. F4/80 can also be seen in a punctuated pattern along the stroma and thecal layers (white arrowheads). C3 is also present in corpus luteum. Individual follicles are circumscribed by dashed lines. All scale bars = 50 μm. Source data are provided as a Source Data file

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