Fig. 7 | Nature Communications

Fig. 7

From: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules

Fig. 7

Binding to endogenous PSD-95. a Western blot analysis of pull-down material from a rat brain lysate using either biotinylated Xph20-ETWV or mScarlet-I as a control. IB stands for immunoblotting. b Western blot analysis of immunoprecipitated material from neuron culture using an anti-His tag antibody in presence or absence of TAT-functionalized Xph20-ETWV. c and d Proteomic analysis of pull-down material from a rat brain lysate using either biotinylated Xph20-ETWV for c or Xph0-ETWV for d using biotinylated mScarlet-I as a non-binding reference (control). The results correspond to triplicates for each series and include proteins identified with a minimum of two peptides. Source data are provided as Source Data file

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