Fig. 2
From: Mouse embryo geometry drives formation of robust signaling gradients through receptor localization
BMP and TGF-β receptors localize at basolateral membrane of hESCs in vitro. a Left column: 3D confocal images of hESC colony stained for BMPR1A (red), tight junction marker ZO-1 (white), and DNA (blue), in lateral (top) and tilted view (bottom). Right column: Zoomed-in lateral images. Scale bar 10 µm. b Plots of BMPR1A (left) and ZO-1 (right) staining intensity along apicobasal axis show BMPR1A localized beneath tight junctions (n = 38 cells from two experiments). c Left column: 3D confocal image of hESC colony stained for DNA (blue), TGFBR1 (red), and ZO-1 (white) in lateral (top) and tilted view (bottom). Right column: Zoomed-in lateral section. d Plots of TGFBR1 (left) and ZO-1 levels (right) against apicobasal axis show that TGFBR1 is localized below tight junctions (n = 51 cells from two experiments). e Confocal image of a hESC expressing TGFBR2-Clover (red), stained for ZO-1 (white). f TGFBR2-Clover (left) and ZO-1 levels (right) against apicobasal axis (n = 4 cells from two experiments). g, h Same as e, f but for BMPR1A-Clover (n = 2 cells from two experiments). i, j Same as e, f but for BMPR1A-HA (n = 3 cells from two experiments). k, l Same as e, f but for BMPR2-Clover (n = 3 cells from two experiments). Yellow arrows in e, g, i, k denote intracellular receptors in secretory pathway. Gray and brown arrows indicate apical and basolateral membranes, respectively. Scale bar 10 µm. Error bars denote SEM
