Fig. 4
From: Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Fast FLIM with a thin PC. a Intensity image of Convallaria majalis rhizome stained with acridine orange, a standard FLIM benchmark (scale bar 100 μm). b Lifetime image from fitting a timing trace of 100 ms exposures (50 μW excitation). c Lifetime image from a single 100 ms acquisition frame. Inset demonstrates the same single frame with 2 ms exposure at high excitation intensity (3 mW—high intensity significantly reduced lifetime in this sample, possibly due to photochemistry). Lifetime images include an intensity mask to show sample structure
