Fig. 3 | Nature Communications

Fig. 3

From: Multi-strategic RNA-seq analysis reveals a high-resolution transcriptional landscape in cotton

Fig. 3

Multiple transcription termination and alternative 3′UTR usage in G. arboreum. a Statistics of transcription end site (TES) per gene. b Cumulative curve of base resolution of the TES cluster signal from PolyA-seq. c The nucleotide composition around the TES. d Statistics of TES usage in genes with multiple TESs including distal- (DT), proximal- (PT) and coding-TESs (CT). e Statistics of effect on RNA (pie chart) and the distribution of change of CDS length due to the use of an alternative TES. f Potential functional RNA elements in the alternative 3′UTRs including RBP binding, U-rich, and RG4 motifs. g Average 3′UTR length across tissues (error bar represents s.e.m.). h Number of genes with alternative TES usage between pairwise tissues. i Genes with changes in APA between ovule (20 DPA) and seed (left), and two representative gene examples (right). The significance levels are indicated by asterisks (*p-value < 0.05; **p-value < 0.01; ***p-value < 0.001), and the median values in box plots are shown. The source data underlying Fig. 3b–h are provided as a Source Data file

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