Fig. 3
From: Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets
TbMscL gating transitions in lipid bilayers. Raw (background uncorrected) PELDOR time-domain traces (left columns) of reconstituted TbMscL in nanodiscs (NDs) or liposomes (Lipos) mutants and distance distributions (right columns). Shaded areas correspond to mean ± 2σ confidence intervals of lipid-reconstituted (red) and solution (blue) mutant distributions as calculated by the DeerAnalysis validation tool (for details see methods), and rainbow color bars indicate the degree of reliability of the measured distance ranges. Rainbow color bars corresponding to the shortest of the two experimental time windows used for the obtained spectra in each case (see Fig. 2), are included. When these time windows are not equal (liposome samples of F88R1, L89R1, R98R1, K100R1, and E102R1), the shortest is that of the reconstituted sample. Distance shift between L89R1 (solution) and (lipid bilayers) suggesting a reversible conformational change in the presence of lateral compression is highlighted by red arrows
