Fig. 2
From: 5-Fluorouracil treatment induces characteristic T>G mutations in human cancer
5-FU mutational pattern and its contribution in human cancer. a Heatmap showing the cosine similarity scores for each de novo extracted signature with the in vitro experimental obtained 5-FU mutation spectrum. NMF H resembles the 5-FU experimental mutation spectrum (cos sim = 0.98) and is further assigned as the “5-FU signature” in the main text. b 5-FU mutation signature showing the mutation type probability for each context-dependent base substitution type. c Box-and whisker plots indicating the relative contribution of the 5-FU signature between 5-FU pretreated and not 5-FU pretreated colon (left) and breast (right) cancer patients with inclusion of the treatment naive cancer cohort. d Box-and whisker plots showing the tumor mutational burden (number of SBSs per Mbp) between 5-FU pretreated and not 5-FU pretreated cancer patients for the colon (left) and breast (right) cancer patients. e Box-and whisker plots showing the 5-FU mutational load between TP53-wild type and TP53-mutant cancers in 5-FU pretreated colon (left) and breast (right) patients. For all plots, a Wilcoxon rank-sum test between every cohort was performed and the P-value is illustrated at the top of the plots. All box-and whiskers plots display the first and the third quartiles (top and bottom of the box), the median (vertical line inside the box), the extremes (whiskers) and, if present, the outliers (single dots)
