Table 1 Lyz–polymer characterization

From: Transforming protein-polymer conjugate purification by tuning protein solubility

Samplea

Estimated DPa

Dh (nm)

Cleaved polymer Mn (kDa)

Đ

Lyz

–

3.6 ± 0.1

–

–

Lyz(5+)pCBMA25

18

7.9 ± 0.4

8.1

1.4

Lyz(5+)pCBMA50

32

11.0 ± 1.0

11.9

1.6

Lyz(5+)pCBMA100

56

13.4 ± 0.9

20.9

1.7

Lyz(5+)pCBMA150

79

15.4 ± 0.8

30.8

1.8

Lyz(5+)pCBMA200

91

16.8 ± 0.8

38.7

1.9

Lyz(5+)pOEGMA25

25

9.2 ± 0.8

17.5

1.7

Lyz(5+)pOEGMA50

43

12.6 ± 1.1

26.9

1.8

Lyz(5+)pOEGMA100

90

20.2 ± 1.7

46.6

1.9

Lyz(5+)pOEGMA150

105

22.2 ± 2.9

53.2

1.8

Lyz(5+)pOEGMA200

164

26.2 ± 3.0

85.4

1.7

  1. Data was collected using a bicinchoninic acid (BCA) assay to estimate degree of polymerization (DP), dynamic light scattering number distribution to measure hydrodynamic diameter (Dh), and acid hydrolysis with gel permeation chromatography (GPC) to calculate number-average molecular mass (Mn) and dispersity (Đ) of cleaved polymer. DLS data are presented as mean number distributions ± 1 standard deviation error bars
  2. aSubscript numbers represent the targeted DP from the ATRP reaction. Estimated DPs are calculated from the BCA assay41. The (5+) represents the number of positively charged initiators on the conjugate
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