Fig. 3
From: Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator
RINGS is unaffected by variable bacterial growth. To test the ability for RINGS analysis to track and integrate growth under variable conditions, IPTG-synchronized PAS715 was back-diluted and grown in liquid culture in the presence or absence of bacteriostatic concentrations of novobiocin. Following 2 h of growth, each culture was washed and split again to be grown under the presence or absence of novobiocin, generating four unique combinations of expected growth and non-growth. The phase of populations sampled at 2 and 4 h were consistent with the expected growth phenotype. In particular, the equivalence of the two populations exposed to one period with and one period without novobiocin, irrespective of order, demonstrates the ability to measure absolute growth during periods of variable growth rate. Mean phase (θ0) from two independent biological replicates is shown. Numbers of colonies analyzed per timepoint are as follows: Rep 1/2 – 0 h: 13/20. 2 h LB: 57/26. 2 h novo: 101/69. 4 h LB-LB: 14/60. 4 h LB-novo: 23/42. 4 h novo-LB: 40/32. 4 h novo-novo: 22/16. Source data are provided as a Source Data file
