Fig. 5
From: Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator
The repressilator 2.0 can be controlled in situ within the mouse gut. a Mice (n = 2 per group) carrying asynchronous PAS715 were provided IPTG or aTc in drinking water to synchronize the repressilator. b RINGS analysis demonstrated the progression from pre- (top) to post-synchronization (bottom). Graph shows histograms of normalized phase count distributions. Numbers are as follows: IPTG1/2—pre: 133/97. Post: 172/206. aTc1/2—pre:149/114. post: 213/193. c. Repressilator in situ synchronization allows RINGS analysis following initial colonization, with bacteria plated on selective plates to assay for repressilator and sponge plasmid retention, or RINGS analysis to follow repressilator phase. After 15 days, provision of streptomycin in drinking water overnight selected remaining PAS715 bacteria to allow isolation and imaging of colonies that had been continually present in the gut. d Plasmid retention (colored samples, left axis) and bacterial abundance (black samples, right axis) data from plating on differentially selective plates. Graph shows individual values and mean line. Numbers are as follows: rep1/2 – 20 h: 772/443. 42 h: 1041/905. 68 h: 1967/1742.102 h: 585/841. e Fluorescence and white light images of two representative colonies of PAS715 isolated 16 days post gavage. Scale bars = 0.1 cm. f RINGS analysis of phase progression 42 h (0 h post sync) -102h (60 h post sync) after gavage. Graphs show histograms of normalized bacterial phase count distributions. For number of colonies analyzed at each timepoint, see corresponding data in g. g Cumulative distribution functions of predicted generations elapsed since gavage. Source data are provided as a Source Data file
