Fig. 2
From: Synthetic protein-conductive membrane nanopores built with DNA
Assembly, purity, dimensions, and membrane-interaction of DNA nanopores NP and NPΔC. a Gel electrophoretic analysis of scaffold strand (ss), nanopores NPΔC and NP without and with detergent SDS, respectively. The position and kilo base pair length of the dsDNA markers are annotated at the sides of the electropherograms. b Representative transmission electron microscopy (TEM) images of negatively stained NPΔC. Scale bar, 50 nm. c Gel electropherogram of NP and NPΔC incubated with no (leftmost lane) or increasing amounts of small unilamellar vesicles (SUVs) ranging in concentrations from 6.9 to 12.5 nM. The upshifted bands of lipid anchor-bearing NP indicate favourable interactions with bilayer membranes. The interaction does not occur for anchor-free NPΔC. The position of the two dsDNA markers with a length of 10 and 1 kbp is given at the right of the gels. d Representative TEM images of negatively stained NP inserted into SUVs. Scale bar, 50 nm. Source data are provided as a Source Data file
