Fig. 3 | Nature Communications

Fig. 3

From: Synthetic protein-conductive membrane nanopores built with DNA

Fig. 3

Electrical recordings confirm the membrane-spanning nature of DNA nanopore NP. a Representative ionic current trace of a single nanopore in 1 M KCl, 10 mM HEPES pH 8.0, and at +20 mV relative to the cis side of the membrane (Fig. 1a). b Histogram of channel conductances obtained from approximately 100 independent single-channel recordings at +20 mV to +50 mV. Red and blue indicate the low and high conductance states of pores, respectively. c Current–voltage (IV) curves displaying the averages and standard deviations from 10 independent single-channel current traces. The low and high conductance states of the pores are shown in blue and red, respectively. Electrical recordings were measured using the Orbit 16 device which is grounded at the cis chamber. Source data are provided as a Source Data file

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