Fig. 6 | Nature Communications

Fig. 6

From: N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis

Fig. 6

CircNSUN2/IGF2BP2/HMGA2 ternary complex stabilized HMGA2 mRNA. a Top, sequence BLAST analysis showing that circNSUN2 directly targets the 3′UTR of HMGA2 with high AU content. Bottom, relative enrichment representing HMGA2 (left panel) and circNSUN2 (right panel) RNA levels associated with circNSUN2 junction compared to control. Data represent mean ± S.D. from three independent experiments; dot plot reflects data points from independent experiment. The P values were determined by a two-tailed unpaired Student’s t test. b CircNSUN2 knockdown in HCT116 cells significantly downregulated HMGA2 mRNA abundance. Data represent mean ± S.D. from three independent experiments; dot plot reflects data points from independent experiment. The P values were determined by a two-way ANOVA. c Left, luciferase mRNA expression of luciferase reporter gene with HMGA2-WT or HMGA2-Mut in control and circNSUN2-knockdown or circNSUN2-overexpression HCT116 cells. Right, relative luciferase activity of luciferase reporter gene with HMGA2-WT or HMGA2-Mut in control and circNSUN2-knockdown or circNSUN2-overexpression HCT116 cells. Data represent mean ± S.D. from three independent experiments; dot plot reflects data points from independent experiment. The P values were determined by a two-tailed unpaired Student’s t test. d IF-FISH assay showing that the colocalization of circNSUN2/IGF2BP2/HMGA2 was decreased upon knockdown of circNSUN2. Scale bar, 10 µm. e Relative enrichment representing the enrichment of HMGA2 associated with truncated IGF2BP2 protein complex compared to an input control. IgG antibody served as a control. Data represent mean ± S.D. from three independent experiments; dot plot reflects data points from independent experiment. f RIP assays showing the association of IGF2BP2 with HMGA2 upon circNSUN2 silencing or overexpression. Data represent mean ± S.D. from three independent experiments; dot plot reflects data points from independent experiment. The P values were determined by a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file

Back to article page