Fig. 4
From: Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
Interaction of UNC-45 proteins and myosin in vitro. a Analytical SEC and SDS-PAGE analysis of UNC-45 and myosin incubated together at 4 °C or at 27 °C and incubated separately at 27 °C and mixed before the SEC run. Misfolded myosin and bound UNC-45 proteins elute earlier from the SEC column, indicated as high molecular weight fractions (HMW, orange bar). b Controls for interaction studies shown in a. BSA or UNC-45 ΔUCS were incubated with myosin at 27 °C. c Melting temperature as determined by CD spectroscopy for the indicated UNC-45 proteins. d Same analysis as in a for the indicated UNC-45 ts-mutants and myosin, incubated together at 27 °C
