Fig. 4
Lipid mediated dimerisation of Vrg4. a Vrg4 dimer observed in the crystal structure with two monoolein lipid molecules (magenta), packed within the dimer interface (dashed line). b SDS–PAGE analysis of Vrg4 reconstituted at different ratios of protein to lipid, showing the presence of a dimer band at ∼ 55 kDa. c Density plot of DPPC lipids around the Vrg4 dimer, in a slice perpendicular to the membrane. Data were gathered using ca. 40 µs of CG MD simulation, and calculated with the VMD volmap plugin. Specific green regions highlight density present at the primary lipid binding region in the dimer interface. The Vrg4 dimer is shown in cartoon and overlaid for reference d Vrg4 dimer observed in the simulation shown in c with four DPPC lipids (green) within the dimer interface shown. e Dominant-negative assay showing the functional unit of Vrg4 is monomeric, and that Vrg4 forms dimers in a liposome. n = 3 (upper panel) or 5 (lower panel) independent experiments, errors shown are s.d. Source data are provided as a Source Data file
