Fig. 5
Identification of short chain lipid binding site. a Density plot of DMPC lipids around the Vrg4 dimer. In addition to the primary lipid binding sites on the dimer interface (labelled 1), secondary binding sites can be observed (labelled 2) (green densities). b Views of Vrg4, with the densities from 4c and 5a shown as coloured mesh. The protein is shown as surface, with each Vrg4 monomer coloured as white or grey. The primary binding site – marked ‘1’ in the top left view – is occupied by both DMPC and DPPC. A secondary site – marked ’2’ – is exclusively occupied by DMPC. c View of the bound DMPC lipid in the secondary binding site, following 30 ns of atomistic simulation. Residues interacting with the lipid are indicated as sticks and labelled and helices are labelled
