Fig. 4
From: A genome-wide assessment of the ancestral neural crest gene regulatory network
Characterisation of a Hox-α2 enhancer and associated transcription. a The Hox-α3/Hox-α2 locus in the sea lamprey germline genome, with merged replicate ATAC-seq coverage tracks from the sea lamprey (Pm) and brook lamprey (Lp) at each developmental stage, as well as RNA-seq coverage tracks from a representative T21 sample indicating directional transcription. Bars below ATAC-seq coverage plots indicate peak regions identified with Macs2. The black boxes highlight two ATAC-seq positive regions within the 9-kb region upstream of the Hox-α2 locus and the dashed boxes highlight bidirectional transcription over these regions. De novo assembled transcripts for the Hox-α2 locus are shown maroon (sense) and dark blue (antisense). b GFP reporter expression in lamprey embryos injected with the element-8.5 kb enhancer-reporter construct at one-cell stage and allowed to grow to indicated stages (observed in 10 out of 149 injected embryos). GFP reporter expression is seen in the neural tube. Scale bars: 200 μm
