Fig. 5
Subpopulations of aHSCs defined by WT1 expression in chronic fibrotic injury in vivo are transcriptionally distinct. a WT1GFP/+ animals were iteratively injured with CCl4 to induce HSC activation and fibrosis prior to isolation of subpopulations defined by WT1(GFP) expression by flow cytometry (n = 3 animals). b Subpopulations of WT1(GFP)-high and WT1(GFP)-intermediate were present within the isolated HSC fraction from injured WT1GFP/+ animals (right panel) but not injured wild-type animals (left, representative plots with population percentages). c A multi-dimensional scaling plot of gene expression after microarray analysis of WT1-positive in vivo activated and WT1-negative in vitro aHSCs demonstrated separation based on WT1(GFP) expression. d Over and underexpressed probes between subpopulations of aHSCs determined by fitting of a linear model. e Hierarchical clustering independently separated subpopulations into groups defined by WT1 expression
