Fig. 2 | Nature Communications

Fig. 2

From: Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy

Fig. 2

Spectroscopic characterization of vibrational sensing effect of SREF with Rh800. a SERF sensing with the nitrile mode of Rh800. b SREF spectra of C≡N band of Rh800 (\(\bar \nu_{{\mathrm{C}} \equiv {\mathrm{N}}}\)) dissolved in different aprotic solvents and mixtures. c \(\bar \nu_{{\mathrm{C}} \equiv {\mathrm{N}}}\) follows a linear relationship with the electric field calculated from the Onsager reaction field theory. d SREF spectra of nitrile in different DMSO–water mixtures. e Plot of \(\bar \nu_{{\mathrm{C}} \equiv {\mathrm{N}}}\) in DMSO–water binary mixture against water volume ratio

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