Fig. 2
From: Rapid single-wavelength lightsheet localization microscopy for clarified tissue
HMSiR blinking signal at different depths with tissue clearing. a The power density can be achieved by our system is plotted with respect to the imaging area that can be covered by lightsheet. The estimation is made by assuming all the power measuring at the back aperture of the excitation objective (20 mW) is transduced into the lightsheet and the imaging area is product of the axial length and the scanning range of the Bessel beam. The maximum axicon lightsheet coverage is 20,000 μm2 (at the power density of 0.1 kW/cm2). Recommended power density ranges for HMSiR activation are indicated in blue. b Representative images of HMSiR blinking signal in phosphate-buffered saline (PBS) and c in Scalview-A2 at the same imaging depth (z = 49 µm) by imaging the HMSiR-labeled membrane GFP in olfactory projection neurons in MZ19-Gal4 > UAS-mCD8::GFP transgenic flies. Images were taken either under a ×25 water dipping objective lens in PBS or a ×25 objective lens specifically designed for use with ScaleView-A2. d Three-dimensional distribution of the signals can be localized in a single volumetric scan. e Representative HMSiR blinking events at four different imaging depths and f overall lateral uncertainty distribution at four different imaging depths in the fly brain cleared with ScaleView-A2. Scale bar = 20 μm in (b–d), 1 μm in (e). Source data are provided as a Source Data file
