Fig. 3
Electron micrographs of silenced and control (Ctrl) cultures. All cultures (pIZ-transformants) were harvested at OD600 = 0.1. a–h Transmission electron micrographs of negatively stained (2% Uranyl acetate) cells of Ctrl in a and SB3×6 cultures in b–d with indicated areas magnified in e–h. Scale bars: a–d: 1 µm; e-h: 200 nm. i–o Envelope morphologies of Ctrl in i and slaB-silenced SB3×6 and SB2 cultures in j–o visualized by transmission electron microscopy of thin sections (contrasted with Uranyl acetate 2% and Lead citrate 2%); Scale bar: 500 nm. p–v Vesicle secretion in Ctrl in p and slaB-silenced SB3×6 and SB2 cells in q–v visualized by transmission electron microscopy of thin sections (contrasted with Uranyl acetate 2% and Lead citrate 2%). Red arrows indicate S-layer. Scale bar: 500 nm
