Fig. 6

Correlation of cell count, size, and DNA content in silenced and control cultures of pIZ-cultures. a Differences in cell counts (cells per ml) determined by flow cytometry (calculated from flow rate) and cell size (FSC, n = 10,000 per biol. repl.) of same cultures harvested at precisely OD₆₀₀ = 0.1 and normalized to Ctrl control. Cell counts: empty bars, cell size: filled bars. Bars in the graph represent the samples in the following order from left to right (for both conditions): control (dark gray); control-CC (light gray); SB3×6 (petrol); SB3×6-CC (light petrol); SB2 (orange); SB2-CC (yellow). Error bars, mean ± SD (three biol. repl.). Asterisks indicate significant differences to Ctrl (two-tailed t test, n ≥ 3, p ≤ 0.018). b Fluorescence histograms of Ctrl, Ctrl-CC, SB3×6, SB3×6-CC pIZ-plasmid cultures harvested at OD₆₀₀ = 0.1 and stained with Hoechst. Peaks represent cell abundances containing one chromosome (1 N), two chromosomes (2 N), and more than two chromosomes (>2 N) in respective cultures (created with Flowing Software 2.5.1). c Percentage of cells containing defined chromosome numbers in each culture (as in b): 1 N (left bars), 2 N (middle bars), >2 N (right bars). Bars in the graph represent the samples in the following order from left to right (in every section): Control (dark gray); control-CC (light gray); SB3×6 (petrol); SB3×6-CC (light petrol). Error bars, mean ± SD (three biol. repl.). Asterisks indicate significant differences to Ctrl (two-tailed t test, n ≥ 3, p ≤ 0.049). Source data are provided⁷⁰