Fig. 7
Infection assays on slaB-silenced and control cultures. a Spotting assay using different dilutions of purified SSV1 virions on cells transformed with pIZ-Ctrl (upper panels) and pIZ-SB3×6 (lower panels), respectively. Two biological replicates presented (the same virion sample was used for the different overlays). b Relative amounts of extracellular virus DNA measured at certain timepoints postinfection (PI) in supernatants of pIZ-SB3×6 (petrol, triangle) and pIZ-Ctrl cultures (dark gray, circles), which have been challenged with SSV1 virions at MOI 0.1. Virus copies were determined by qPCR using D-291 FW/RV primers (Supplementary Table 1) and normalized to cell count in the individual samples and to the initial virus added. Percentage in relation to timepoint 10 min PI is shown. Error bars, mean ± SD (three biol. and techn. replicates). Asterisks indicate significant differences to Ctrl (two-tailed t test, n ≥ 3, p ≤ 0.0105). c Fold change of increase of virus DNA inside cells in same samples as in b. Virus DNA measurement was conducted as in b; error bars are defined as in b. Asterisks indicate significant differences to Ctrl (two-tailed t test, n ≥ 3, p ≤ 0.0083). Source data are provided70
