Fig. 2

TRPML1 is permeable to physiological levels of Zn²⁺. a Representative traces of FluoZin-3-stained HeLa cells either un-transfected (no TRPML1 OE, green), or expressing dominant negative (TRPML1^DDKK, magenta) or wild-type (TRPML1^WT, blue) mCherry-TRPML1. Cells were treated with 100 µM ZnCl₂ at 0 s then 50 µM ML-SA1 at 300 s. b Mean slope (±s.e.m.) of FluoZin-3 signal ((∆F/F₀)/min) for 60 s following ML-SA1 addition for TRPML1^WT (blue, n = 11 cells), no TRPML1 OE (green, n = 18), TRPML1^DDKK (magenta, n = 10), TRPML1^WT ZnCl₂ only (light gray, n = 11), TRPML1^WT ML-SA1 only (n = 11). One-way ANOVA, with post-hoc Tukey HSD. c Representative traces of cells expressing GZnP3 alone (no TRPML1 OE, green), or co-transfected with dominant negative (TRPML1^DDKK, magenta) or wild-type (TRPML1^WT, blue) mCherry-TRPML1. Cells were treated as described in a. d Mean slope (±s.e.m.) of GZnP3 signal ((∆F/F₀)/min) for 60 s following ML-SA1 addition for TRPML1^WT (blue, n = 11 cells), no TRPML1 OE (green, n = 11), TRPML1^DDKK (magenta, n = 14), TRPML1^WT ZnCl₂ only (light gray, n = 12), TRPML1^WT ML-SA1 only (n = 11). One-way ANOVA, with post-hoc Tukey HSD. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. p-values displayed in italics above corresponding comparisons, where applicable. Source data are provided as a Source Data file