Fig. 3
From: YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression
YTHDF1 depletion inhibits de novo ADC progression. a Xenograft tumor masses harvested from indicated A549 cell lines after tumors had grown for 42 days. b, c YTHDF1 elimination inhibits xenograft tumor weight (b) and volumes (c) in male nude mice. d Representative IHC images showing YTHDF1 knockdown in xenografts. Scale bar: 50 μm. e A scheme of Adeno-Cre treatment-induced KP and KPY mouse models. f YTHDF1 is upregulated in ADC from KP mice compared with the complete loss of YTHDF1 in KPY mouse tumors. Scale bar, 50 μm. g, h Representative images of H&E-stained lung sections of KP or KPY mice at 12 weeks after viral administration (g). Quantification data for the tumor numbers and tumor size (≥1 mm2) in KP or KPY mice (h). Scale bars are indicated in each image. T Tumor tissues. i Representative images of positive staining of Napsin A and TTF-1 for KP mice ADC tumors. Scale bar, 50 μm. j, k Representative images of Ki67 and CC3 immunostaining (j) of lung tumors and statistical analyses of the Ki67- and CC3-positive index in the indicated genotype (k), respectively. CC3: cleaved caspase 3. Scale bar, 50 μm. Means ± SEM, *P < 0.05; t-test
