Fig. 5
From: Regulation of the ER stress response by a mitochondrial microprotein
PIGBOS shows no effect on modulation of ER-mitochondria contacts. a Representative transmission electron microscopy images from WT and PIGBOS-KO U2OS cells showed no remarkable differences in ER-mitochondria contacts. ER and mitochondria contact sites are indicated by white arrows. b Quantitation of the normalized ER-mitochondria contact coefficient (ERMICC) did not identify a significant difference between ERMICC of WT vs. PIGBOS-KO U2OS cells. Data are collected from two independent experiments and pooled from 43 WT mitochondria (eight cells), and 62 PIGBOS-KO mitochondria (seven cells) and the bar graph is the ERMICC ± s.e.m. with the p-value calculated using two tailed unpaired t-test with all data points included in the calculation. Source data for Fig. 5b are provided as a Source Data file
