Fig. 1

Ageing upregulates mir-83 in the intestine through hsf-1. a qRT-PCR of miR-83 in the wild type (WT) worms of indicated ages. The levels of miR-83 were normalized against the one at day 1. n = 7, 4, 3, 3, and 3 independent experiments for samples of day 1, 4, 7, 14, and 21, respectively. b Immunoblots of mir-83p::GFP at indicated ages. Blots against α-tubulin and day 1 sample serve as controls for loading and normalization respectively. n = 3 independent experiments. c Representative images of mir-83p::gfp transgenic animals at indicated ages. n: neuron, i: intestine. d GFP intensity of mir-83p::gfp in the intestine and head neurons at day 1, day 4, and day 7 of adulthood. Samples of day 1 serve as controls for normalization. n = 3 independent experiments containing at least 41 worms. e qRT-PCR of miR-83 in WT worms treated with luc2 or hsf-1 RNAi. RNAi treatment was performed from hatching and worms were examined at indicated ages. Samples of day 1 serve as controls for normalization. n = 3 independent experiments. f Expression of mir-83p::gfp under luc2 or hsf-1 RNAi. Worms were treated with RNAi from hatching and examined for GFP intensity at indicated ages. day 1 worms subjected to luc2 RNAi serve as control for normalization. n = 3 independent experiments containing at least 39 worms. Scale bars: 100 μm. Statistical significance was calculated by One-way ANOVA in (a and b), or Two-way ANOVA in d–f. ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file