Fig. 4

mir-83 impairs autophagy in the body wall muscle with ageing. a GFP::LGG-1 puncta (arrow heads) in the body wall muscle (BWM) (dashed lines) of WT worms and mir-83(-) mutants at indicated ages. n = 3 independent experiments containing at least 25 worms. b mCherry::GFP::LGG-1 signals in the BWM (dashed lines) of WT worms and mir-83(-) mutants at indicated ages. White arrow heads denote the AL puncta and yellow arrows denote the AP puncta. n = 3 independent experiments containing at least 29 worms. c GFP::LGG-1 puncta (arrow heads) in the BWM (dashed lines) of indicated strains at day 1 of adulthood post the treatment of 5 mM chloroquine (CQ) or a mock treatment (Ctrl). n = 3 independent experiments containing at least 28 worms. d SQST-1::GFP puncta (arrow heads) in the BWM (dashed lines) of indicated strains. n = 5, 5, 4, and 4 independent experiments for samples of day 1, 3, 5, and 7, respectively. Each sample contains at least 106 worms. e The number of GFP::LGG-1 puncta (arrow heads) in WT worms and mir-83(-) mutants with or without an intestine-specific transgene of mir-83 (IntOE). Dashed lines denote BWM cells. n = 3 independent experiments containing at least 23 worms. f The intestine-specific expression of mir-83 abolishes the increased ALs (white arrow heads) in BWM cells (dashed lines) of mir-83(-) mutants. Note that APs (yellow arrows) were increased in WT worms due to a block of the autophagy flux. n = 4 independent experiments containing at least 19 worms. Scale bars: 10 μm. Statistical significance was calculated by Poisson regression. ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file