Fig. 4
From: B-1a cells acquire their unique characteristics by bypassing the pre-BCR selection stage
B-1a cells are efficiently generated in surrogate light chain-deficient mice. a Representative flow-cytometry plots of peritoneal cavity cells from wild-type and Igll1−/− mice. B-2 and B-1 cell gates are displayed as a percentage of CD19+ cells (left). B-1a and B-1b cell gates are displayed as a percentage of B-1 cells (right). b The total cell numbers of B-2, B-1b, and B-1a cells were calculated from the peritoneal cavity of these mice. The middle line in the boxplots represents the median. The box shows first and third quartiles, and the whiskers represent the max and min values. Each dot represents cells from a different individual mouse. P-values were calculated using a two-tailed T test. Source data are provided as Source Data File. c Igll1−/− mice maintain both functionally distinct PC1lo and PC1hi subsets of B-1a cells. Representative flow-cytometry plots of peritoneal cavity cells from wild-type and Igll1−/− mice. B-2 and B-1 cell gates are displayed as a percentage of CD19+ cells (left). PC1lo and PC1hi B-1a cells are displayed as a percentage of B-1a cells (right). A PC1 FMO (fluorescence minus one) was used to define the gate
