Fig. 6
From: B-1a cells acquire their unique characteristics by bypassing the pre-BCR selection stage
Constitutive phospho-STAT5 signaling selectively inhibits B-1a cell development. a Representative flow-cytometry plots of peritoneal cavity cells from wild-type and Stat5b-CA mice. B-2 (CD19+B220hi) and B-1 (CD19+B220lo) cell gates are displayed as a percentage of CD19+ cells (left). B-1a (CD19+B220loCD5+) and B-1b (CD19+B220loCD5−) cell gates are displayed as a percentage of the B-1 cells (right). b Graphs display the total cell numbers of B-2, B-1b, and B-1a cells from the peritoneal cavity of wild-type and Stat5b-CA mice. Each dot represents an individual mouse, and lines connect pairs of littermates from three different litters of mice. c, d SLC-independent B-1a cells express receptors with phosphatidylcholine (PtC)-specific VH12 gene rearrangements. Peritoneal cavity B-1a cells from wild-type and Igll1−/− mice highlight the proportion of B-1a cells that are either c VH12+, PtC+ or d PtC+. The left side shows representative flow-cytometry plots. The right side is a graphical summary of the mice from all the experiments. Each dot represents an individual mouse and lines connect pairs of littermates. P-values were calculated using a two-tailed T -test. Source data are provided as Source Data File
