Fig. 1 | Nature Communications

Fig. 1

From: Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

Fig. 1

Analysis of human deletion alleles for flanking microhomologies (µHs). a Overview of double-strand break (DSB) DNA repair pathways and their outcomes. homology-directed repair (HDR), classical non-homologous end joining (c-NHEJ), microhomology-mediated end joining (MMEJ). b Schematic of the MHcut tool used to identify the microhomologous sequences. microhomologies (µHs, green) or nested µHs (red), SpCas9 PAM (underline) and DSB location (pink bolt). c Numbers for variant data input and result of filters applied to the MHcut tool output. bp, base pairs. d Distribution of µH-flanked deletion variants by deletion size. e Distribution of µH-flanked deletion variants by µH length. f µH-flanked deletion variants plotted by µH distance with µH length indicated by fill color. g Percentage of protein-coding genes with µH-flanked deletion variant in exonic coding sequences

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