Fig. 4

TM3 and TM4 in nhTMEM16 are locked in hydrophobic interactions mediated by the L302/I343/L347 triad of residues. a Two views, related by 90◦ rotation around the membrane normal, of the groove region in the WT nhTMEM16 protein, show the locations of L302, I343, and L347 (in space fill and labeled). Helices TM3-TM6 that line the groove are labeled, and colored red, blue, white and gray, respectively. b Histograms of minimal distance between residue pairs L302 and I343 (in red), and L302 and L347 (in black), in the simulation trajectories of WT nhTMEM16 (see also Supplementary Fig. 6)