Fig. 3
From: Identification and characterization of Cardiac Glycosides as senolytic compounds
Mechanism of action of Digoxin-induced senolysis. a Diagram showing the activity of Digoxin. b Relative cell viability (%) of proliferative (green) or senescence (red) A549 cells overexpressing human or mouse ATP1A1 or Atp1a1, respectively, and treated with Digoxin or Navitoclax. Statistical analysis with Anova with Tuckey test: ***p < 0.001; *p < 0.05; c and mRNA expression (relative to GAPDH) of ATP1A1 (left) and Atp1a1 (right). n = 3 independent experiments for both d Membrane potential determination of proliferative (green) or senescence (red) A549 cells treated with Digoxin and KCl, using fluorescent probe DiBAC4(3). n = 10 biologically independent samples. Statistical significance by two-tailed Student's t-test: **p < 0.01; *p < 0.05. e Relative cell viability (%) of proliferative (green) or senescence (red) A549 cells treated with Digoxin and KCl. n = 7 biologically independent samples. Statistical analysis with Anova with Tuckey test: **p < 0.01. f Determination of the relative intracellular H+concentration in proliferative (green) or senescence (red) A549 cells treated or not with Digoxin. n = 4 biologically independent samples. Statistical significance by two-tailed Student's t-test: ***p < 0.001. g Relative cell viability (%) of proliferative (green) or senescence (red) A549 cells treated or not with Amiloride. n = 3 biologically independent samples. Statistical significance by two-tailed Student's t-test: ***p < 0.001. h Relative mRNA expression of SLC9A1 in proliferative (green) or senescence (red) A549 cells overexpressing or not SLC9A1 as indicated. n = 3 biologically independent samples. Statistical significance by two-tailed Student's t-test: **p < 0.01; *p < 0.05. i Relative cell viability (%) of proliferative (green) or senescence (red) A549 cells treated with Digoxin and exogenously expressing SLC9A1 or GFP. n = 3 independent experiments. Statistical significance by two-tailed Student's t-test: **p < 0.01; n.s. not significant. All data correspond to the average ± s.d. Source data for these experiments are provided as a Source Data file
