Fig. 2
From: Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
Key junctional, linker and motif sequences for cys-trapped human pMHCII and mKIH-based mouse pMHCII. a Key amino acid sequences of human pMHCII molecules encoding a cys-trapped IGRP13–25/DRA1*0101/DRB1*0301 pMHCII heterodimerized via c-jun/c-fos leucine zippers (also referred to “conventional”). ‘…’ is used to indicate that corresponding intervening amino acid sequences are not shown, as they are publicly available. Residues in red are mutated and the original residue and its position are indicated immediately below. b Key amino acid sequences of a mouse pMHCII molecule encoding BDC2.5mi/IAαd/IAβg7 heterodimerized using a carboxyterminal mouse IgG1-Fc-based KIH
