Fig. 4
From: Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
Cartoons depicting the structure of the KIH-based pMHCII constructs or specific domains. Left: primary structure of a Cys-trapped KIH-based pMHCII heterodimer. Top right: secondary structure of the peptide-binding domain loaded with a peptide bound to the MHCII molecule on a specific register via a disulfide bridge between the carboxyterminal end of the peptide and a complementary Cys on the MHCII α chain. Bottom right: predicted quaternary structure of the KIH Fc portion of the KIH-based constructs and the key amino acid substitutions that were used to promote KIH-based heterodimerization
