Fig. 8
From: Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
TCR signaling potency of pMHC-NPs as a function of pMHCII molarity or NP number. a, b Luciferase activity induced by NPs coated with c-jun/c-fos- (‘conv’) or KIH-based BDC2.5mi/IAg7 monomers (normalized to that induced by soluble anti-CD3ε mAb) on Jurkat cells co-expressing mouse CD4, a BDC2.5mi/IAg7-specific TCR and an NFAT-driven luciferase reporter. Data correspond to main Fig. 7b but normalized by molar concentration of pMHCII or NP number. c, d Luciferase activity induced by NPs coated with KIH-based BDC2.5mi/IAg7 pMHCIIs carrying a mouse or a human Fc-based KIH (normalized to that induced by soluble anti-CD3ε mAb) on Jurkat cells co-expressing mouse CD4, a BDC2.5mi/IAg7-specific TCR and an NFAT-driven luciferase reporter. Data correspond to main Fig. 7e but normalized by molar concentration of pMHCII or NP number. e, f Luciferase activity induced by NPs coated with c-jun/c-fos-based (‘conv’), Cys-trapped IGRP13–25/DR3 pMHCs vs. NPs coated with non-Cys-trapped KIH-based IGRP13–25/DR3 coated at three different valencies on Jurkat cells co-expressing human CD4, an IGRP13–25/DR3-specific TCR and an NFAT-driven luciferase reporter. Data correspond to main Fig. 9d but normalized by molar concentration of pMHCII or NP number. g, h Luciferase activity induced by NPs coated with c-jun/c-fos-based/Cys-trapped or KIH-based/Cys-trapped IGRP13–25/DR3 monomers vs. their non-Cys-trapped counterparts on Jurkat cells co-expressing human CD4, an IGRP13–25/DR3-specific TCR and an NFAT-driven luciferase reporter. Data correspond to main Fig. 9e but normalized by molar concentration of pMHCII or NP number. Data correspond to mean ± SEM of triplicates. Source data are provided as a Source Data File
