Fig. 6 | Nature Communications

Fig. 6

From: An anionic, endosome-escaping polymer to potentiate intracellular delivery of cationic peptides, biomacromolecules, and nanoparticles

Fig. 6

PPAA pretreatment enhances cationic nanoparticle uptake and bioactivity. a Schematic representation of cationic, amine-surface modified 200 nm polystyrene nanoparticles, synthesis of Cy5-labeled DNA-loaded D-DPB polymeric micellar nanoparticles, and sequential delivery of these nanoparticle formulations following PPAA pretreatment. The effects of dose-dependent PPAA pretreatment on the uptake of b cationic polysterene nanoparticles and c Cy5-labeled DNA-loaded D-DPB nanoparticles in A7r5 cells; *p < 0.05, vs. treatment with nanoparticles alone (i.e., 0 nM PPAA). d The effects of dose-dependent PPAA pretreatment on luciferase gene silencing in luciferase expressing A7r5s; *p < 0.05, **p < 0.01, vs. D-DPB nanoparticles loaded with scrambled siRNA (scramble). For b–d, statistical analyses were performed with a one-way ANOVA followed by a Tukey’s post hoc test. Data are presented as means ± SEM. e PPAA dose-dependent effects on uptake vs. bioactivity

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