Fig. 6
From: Split intein-mediated selection of cells containing two plasmids using a single antibiotic
SiMPl plasmids increase production of the non-ribosomal peptide indigoidine. a Images of BAP1 cells producing indigoidine on plate (left) and in liquid culture (right). b Schematic of indigoidine biosynthesis. AGln, Adenylation (A) domain specific for glutamine; Ox, oxidation domain embedded within the A domain; PCP, peptidyl carrier protein domain. The PCP domain is depicted with the PPant arm attached to it; TE, thioesterase domain. c Schematic representation of the truncated BpsA (BpsAΔTE) and the externally supplied TE domain used in this study. d, e Schematic of the plasmids used in (f). f Bar graph showing the absorbance at 610 nm of overnight expression cultures in 85% DMSO of BAP1 cells expressing the indicated constructs from the indicated plasmids induced with 100 μM IPTG at 18 °C. Values were normalized to the value of the control (full-length BpsA expressed from pTrc99a) and represent mean (± standard error of the mean) of three biological replicates. ns, not significant (two-tailed, homoscedastic Student’s t test).; double asterisk (**), p-value < 0.01 (two-tailed, heteroscedastic Student’s t test). pCDF, plasmid shown in (e). Source data are provided as a Source Data file
