Fig. 8
From: Split intein-mediated selection of cells containing two plasmids using a single antibiotic
SiMPl can be used to select human T cells with puromycin. a Schematic of the SiMPl lentiviral vectors encoding mTagBFP2 and ZsGreen1 where the puromycin acetyltransferase is split at position V82:E83. b Schematic of the workflow. c T cells selected on puromycin after transduction with the two lentiviruses in (a) are 100% positive for mTagBFP2 and ZsGreen1. Left panel, representative flow cytometric analysis of mTagBFP2 and ZsGreen1 expression in living cells gated using forward versus side scatter. The numbers indicate the percentage of mTagBFP2- and/or ZsGreen1-positive cells in the indicated quadrants. Right panel, stacked bar chart showing the percentage of cells in the four quadrants (lower left, ZsGreen1− mTagBFP2−; lower right, ZsGreen1+ mTagBFP2−; upper left, ZsGreen1− mTagBFP2+; upper right, ZsGreen1+ mTagBFP2+) at the indicated conditions. Values represent mean (± standard deviation) of three independent experiments. Standard bar graphs with individual data points are shown in Supplementary Fig. 20. Source data are provided as a Source Data file
