Fig. 1
From: Structural insights of human mitofusin-2 into mitochondrial fusion and CMT2A onset
Overall structure of truncated MFN2IM a Schematic representation showing the organization of MFN2IM based on full-length MFN2. G domain, GTPase domain; HD1/2, helical domain 1/2; T, transmembrane region. Elements for MFN2IM are assigned according to the structure. Borders of each element are indicated by residue numbers. b Structure of nucleotide-free MFN2IM. α-helices of HD1 are differentially colored to specify their distribution on the primary structure as in a. c Details of hinge 2 of MFN2IM in nucleotide-free state. d Structural comparison between Trp260 in MFN2IM (pink) and Trp239 in MFN1IM (gray) in nucleotide-free states. e The C terminus of ɑ4H physically contacts the G domain. Nucleotide-free MFN2IM is illustrated here. f Binding affinities (dissociation constant, Kd) to guanine nucleotides for nucleotide-free MFN2IM and MFN1IM measured by isothermal titration calorimetry (ITC)
