Fig. 2
From: Structural insights of human mitofusin-2 into mitochondrial fusion and CMT2A onset
MFN2 is a weak GTPase. a GTP turnover rates of MFN2IM and MFN1IM were measured at seven different protein concentrations. Error bars indicate s.d. (n = 3). b GDP off-rates of MFN2IM and MFN1IM. A representative trace from three independent experiments is shown for each sample. c Sequence alignment of the P-loop and switch I of mitofusins from various species. Conventional motifs/residues are highlighted in green. Residues that differ between human MFN1 and MFN2 and may be responsible for GTP binding and hydrolysis are highlighted in red. hs Homo sapiens, bt Bos taurus, mm Mus musculus,; dm Drosophila melanogaster, sc Saccharomyces cerevisiae, np Nostoc punctiforme. d GTP turnover rates of wild-type human (hs) and mouse (mm)MFN1IM/MFN2IM, and indicated mutants. Error bars indicate s.d. (n = 3). e GDP off-rates of MFN2IM(T129I) and MFN1IM(I108T). Source data are provided as a Source Data file
